Pancreatic phospholipase A2: isolation of the human gene and cDNAs from porcine pancreas and human lung.

@article{Seilhamer1986PancreaticPA,
  title={Pancreatic phospholipase A2: isolation of the human gene and cDNAs from porcine pancreas and human lung.},
  author={J. J. Seilhamer and Tony Randall and Miles Yamanaka and Linda Johnson},
  journal={DNA},
  year={1986},
  volume={5 6},
  pages={519-27}
}
Oligonucleotide probes synthesized using the published protein sequence for porcine pancreatic phospholipase A2 (PLA2; Puijk et al., 1977), were used to screen a cDNA library made from porcine pancreas. One resulting clone which encoded the entire porcine PLA2 enzyme was then used to screen various human cDNA and gene libraries. A 562-bp cDNA clone from human lung and two overlapping genomic clones encoding the identical transcript were obtained. These clones encoded a 126-residue peptide… CONTINUE READING

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