Pancreatic expression of human insulin gene in transgenic mice.

  title={Pancreatic expression of human insulin gene in transgenic mice.},
  author={Danielle Bucchini and M A Ripoche and Marie-georges Stinnakre and Pierrette Desbois and Patrick Lor{\`e}s and E Monthioux and J Absil and Jean Antoine Lepesant and R. Pictet and Jacques Jami},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={83 8},
We have investigated the possibility of obtaining integration and expression of a native human gene in transgenic mice. An 11-kilobase (kb) human chromosomal DNA fragment including the insulin gene (1430 base pairs) was microinjected into fertilized mouse eggs. This fragment was present in the genomic DNA of several developing animals. One transgenic mouse and its progeny were analyzed for expression of the foreign gene. Synthesis and release of human insulin was revealed by detection of the… CONTINUE READING

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