Pan-Pathway Based Interaction Profiling of FDA-Approved Nucleoside and Nucleobase Analogs with Enzymes of the Human Nucleotide Metabolism

@inproceedings{Egeblad2012PanPathwayBI,
  title={Pan-Pathway Based Interaction Profiling of FDA-Approved Nucleoside and Nucleobase Analogs with Enzymes of the Human Nucleotide Metabolism},
  author={Louise Egeblad and Martin Welin and Susanne Flodin and Susanne Gr{\"a}slund and Liya Wang and Jan Marie Rene Balzarini and Staffan K Eriksson and Paer Nordlund},
  booktitle={PloS one},
  year={2012}
}
To identify interactions a nucleoside analog library (NAL) consisting of 45 FDA-approved nucleoside analogs was screened against 23 enzymes of the human nucleotide metabolism using a thermal shift assay. The method was validated with deoxycytidine kinase; eight interactions known from the literature were detected and five additional interactions were revealed after the addition of ATP, the second substrate. The NAL screening gave relatively few significant hits, supporting a low rate of "off… CONTINUE READING

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