Palmitoylation controls the dynamics of budding-yeast heterochromatin via the telomere-binding protein Rif1

  title={Palmitoylation controls the dynamics of budding-yeast heterochromatin via the telomere-binding protein Rif1},
  author={Sookhee Park and Erin E. Patterson and Jenel Cobb and Anjon Audhya and Marc R. Gartenberg and Catherine A. Fox},
  journal={Proceedings of the National Academy of Sciences},
  pages={14572 - 14577}
The posttranslational addition of palmitate to cysteines occurs ubiquitously in eukaryotic cells, where it functions in anchoring target proteins to membranes and in vesicular trafficking. Here we show that the Saccharomyces cerevisiae palmitoyltransferase Pfa4 enhanced heterochromatin formation at the cryptic mating-type loci HMR and HML via Rif1, a telomere regulatory protein. Acylated Rif1 was detected in extracts from wild-type but not pfa4Δ mutant cells. In a pfa4Δ mutant, Rif1-GFP… 

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