Palmitate uptake by cultured hepatocytes: albumin binding and stagnant layer phenomena.

@article{Burczynski1989PalmitateUB,
  title={Palmitate uptake by cultured hepatocytes: albumin binding and stagnant layer phenomena.},
  author={F. Burczynski and Z. Cai and J. B. Moran and E. L. Forker},
  journal={The American journal of physiology},
  year={1989},
  volume={257 4 Pt 1},
  pages={
          G584-93
        }
}
We compared uptake of palmitate by hepatocyte monolayers with uptake by polyethylene membranes under conditions of identical binding and stirring. Hepatocytes and polyethylene display similar clearances when the fatty acid is free, reflecting partial rate limitation by diffusion across the unstirred water layer. When palmitate is 99.8% bound to albumin, however, hepatocytes clear free fatty acid about seven times faster than does polyethylene. We analyzed the uptake of palmitate by polyethylene… Expand
View on PubMed
doi.org
39 Citations
Highly Influential Citations
1
Background Citations
14
Methods Citations
3

39 Citations

Citation Type

Citation Type

Effect of binding protein surface charge on palmitate uptake by hepatocyte suspensions
  • 21
Palmitate transport by rat renal basolateral membrane vesicles in the presence of albumin.
  • M. Trimble
  • Chemistry, Medicine
  • Journal of the American Society of Nephrology : JASN
  • 1993
  • 6
Oleate uptake kinetics in the perfused rat liver are consistent with pseudofacilitation by albumin.
  • 16
Uptake of organic anions by hepatocyte monolayers: codiffusion versus facilitated dissociation.
  • 5
Cytosolic fatty acid binding protein enhances rat hepatocyte [3H]palmitate uptake.
  • 17
Hepatocyte [3H]-palmitate uptake: effect of albumin surface charge modification.
  • 24
Assessing the cellular transmembrane electrical potential difference on the hepatic uptake of palmitate
Is there facilitated uptake of fatty acids by the liver? Interpretation and analysis of experimental data.
  • 22
The Phenomenon of Albumin-Mediated Hepatic Uptake of Organic Anion Transport Polypeptide Substrates: Prediction of the In Vivo Uptake Clearance from the In Vitro Uptake by Isolated Hepatocytes Using a Facilitated-Dissociation Model
  • 31
  • PDF
Effect of nitric oxide on albumin-palmitate binding.
  • 14
...
1
2
3
4
...