Pairwise selection assembly for sequence-independent construction of long-length DNA

  title={Pairwise selection assembly for sequence-independent construction of long-length DNA},
  author={William J. Blake and Brad A. Chapman and Anuradha Zindal and Michael E. Lee and Shaun M. Lippow and Brian M. Baynes},
  journal={Nucleic Acids Research},
  pages={2594 - 2602}
The engineering of biological components has been facilitated by de novo synthesis of gene-length DNA. Biological engineering at the level of pathways and genomes, however, requires a scalable and cost-effective assembly of DNA molecules that are longer than ∼10 kb, and this remains a challenge. Here we present the development of pairwise selection assembly (PSA), a process that involves hierarchical construction of long-length DNA through the use of a standard set of components and operations… 

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