Paired image- and FACS-based toxicity assays for high content screening of spheroid-type tumor cell cultures

@inproceedings{Trumpi2015PairedIA,
  title={Paired image- and FACS-based toxicity assays for high content screening of spheroid-type tumor cell cultures},
  author={Kari Trumpi and David A. Egan and Thomas T. Vellinga and Inne H. M. Borel Rinkes and Onno W. Kranenburg},
  booktitle={FEBS open bio},
  year={2015}
}
Novel spheroid-type tumor cell cultures directly isolated from patients' tumors preserve tumor characteristics better than traditionally grown cell lines. However, such cultures are not generally used for high-throughput toxicity drug screens. In addition, the assays that are commonly used to assess drug-induced toxicity in such screens usually measure a proxy for cell viability such as mitochondrial activity or ATP-content per culture well, rather than actual cell death. This generates… CONTINUE READING
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