Packaging specific segments of the Salmonella chromosome with locked-in Mud-P22 prophages.

  title={Packaging specific segments of the Salmonella chromosome with locked-in Mud-P22 prophages.},
  author={Philip A. Youderian and P Sugiono and Ken Brewer and N. Patrick Higgins and Thomas Elliott},
  volume={118 4},
Hybrid genetic elements, Mud-P and Mud-Q (collectively, Mud-P22s), have been constructed that carry two-thirds of the temperate Salmonella phage P22 genome sandwiched between the ends of transposon Mu. Insertions of these elements in the Salmonella chromosome generate locked-in P22 prophages that cannot excise. Upon induction (as a consequence of the inactivation of P22 c2 repressor), a locked-in prophage replicates its DNA in situ, resulting in the amplification of neighboring regions of the… CONTINUE READING
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