PYP‐1, inorganic pyrophosphatase, is required for larval development and intestinal function in C. elegans

  title={PYP‐1, inorganic pyrophosphatase, is required for larval development and intestinal function in C. elegans},
  author={Kyung Min Ko and Wonhae Lee and Jae-Ran Yu and Joohong Ahnn},
  journal={FEBS Letters},

Structural and Biochemical Characterization of Apicomplexan Inorganic Pyrophosphatases

The results show that PfPPase is a cytosolic enzyme whose gene expression is upregulated during parasite asexual stages and its crystal structures reveal unusual and unprecedented dimeric organizations and provide new fundamental insights into the variety of oligomeric assemblies possible in eukaryotic inorganic PPases.

Regulation of pyrophosphate levels by H+-PPase is central for proper resumption of early plant development

Evidence is presented of the growth-boosting effects of removing PPi in later stages of plant vegetative development, and the biological role of PPases and their potential applications in different disciplines and in various organisms are discussed.

Crystal Structure of Inorganic Pyrophosphatase From Schistosoma japonicum Reveals the Mechanism of Chemicals and Substrate Inhibition

The results suggest that the SjPPase possesses PPi hydrolysis activity, and the activity declines with increased MDP or NaF concentration, however, the enzyme shows unexpected substrate inhibition properties.

Tissue-targeted inorganic pyrophosphate hydrolysis in a fugu5 mutant reveals that excess inorganic pyrophosphate triggers developmental defects in a cell-autonomous manner

This study provides a conceptual framework to unveil metabolic fluctuations within leaf tissues with high spatio–temporal resolution and suggests that excess PPi exerts its inhibitory effect in planta in the early stages of seedling establishment in a tissue- and cell-autonomous manner.

Excess Pyrophosphate Restrains Pavement Cell Morphogenesis and Alters Organ Flatness in Arabidopsis thaliana

The data suggest that PPi homeostasis is a prerequisite for proper pavement cell morphogenesis, epidermal growth and development, and organ flattening, as well as supporting a link between PPi and MT dynamics.

Pyrophosphate modulates plant stress responses via SUMOylation

It is shown here, that both cold- and heat stress sensitivity of fugu5 mutants lacking the major H+-PPase isoform AVP1 is correlated with reduced SUMOylation and this results provide a mechanistic explanation for the beneficial effects of AVP 1 overexpression in plants.

Identification of Critical Elements for Regulation of Inorganic Pyrophosphatase (PPA1) in MCF7 Breast Cancer Cells

PPA1 is positively regulated by Sp1 and p300 coactivates Sp1 induced PPA1 promoter activity and histone acetylation/deacetylation may contribute to a local chromatin remodeling across the PPA2 promoter.

Lats kinase is involved in the intestinal apical membrane integrity in the nematode Caenorhabditis elegans

The data presented here suggest that Lats kinase plays important roles in the integrity of the apical membrane structure of intestinal cells.

Pyrophosphate inhibits gluconeogenesis by restricting UDP-glucose formation in vivo

The results indicate that UGP-Glc pyrophosphorylase is the major target when excess PPi exerts inhibitory effects in vivo, and support the notion that H+-PPase plays a pivotal role in cytosolic PPi homeostasis in plant cells.



Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites.

The studies provide evidence that the PPases are novel enzymes in the roundworm Ascaris, and may have crucial role in the development and molting process, and are potent inhibitor of both soluble- and membrane-bound H+-PPases.

Genetic analysis of lysosomal trafficking in Caenorhabditis elegans.

The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that are shown to be lysosome-related organelles, and the glo mutants provide a simple model system for the analysis of lysOSome- related organelle biogenesis in animal cells.

Evolutionary conservation of the active site of soluble inorganic pyrophosphatase.

Pyrophosphatase of the Roundworm Ascaris suum Plays an Essential Role in the Worm's Molting and Development

It is established that AsPPase has a critical role in the molting and development of Ascaris roundworms and the potential of As PPase for use as a candidate vaccine against ascariasis is suggested.

Calreticulin, a calcium-binding molecular chaperone, is required for stress response and fertility in Caenorhabditis elegans.

The functional characterization of Caenorhabditis elegans calreticulin (crt-1) is reported, suggesting that CRT-1 may be important for stress-induced chaperoning function in C. elegans.

Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is involved in movement, fertility, egg laying, and growth in Caenorhabditis elegans.

Double mutants of cnb-1 and unc-43(gf) displayed an apparent synergistic severity of movement and egg-laying defects, suggesting that calcineurin may have an antagonistic role in CaMKII-regulated phosphorylation signaling pathways in C. elegans.