PURIFICATION AND PROPERTIES OF DESOXYRIBONUCLEASE ISOLATED FROM BEEF PANCREAS

@article{Mccarty1946PURIFICATIONAP,
  title={PURIFICATION AND PROPERTIES OF DESOXYRIBONUCLEASE ISOLATED FROM BEEF PANCREAS},
  author={Maclyn Mccarty},
  journal={The Journal of General Physiology},
  year={1946},
  volume={29},
  pages={123 - 139}
}
  • M. Mccarty
  • Published 20 January 1946
  • Biology, Chemistry
  • The Journal of General Physiology
1. A method is described for the isolation and purification of desoxyribonuclease from a 0.25 N sulfuric acid extract of beef pancreas. The activity of the enzyme is measured by a viscosimetric method using sodium desoxyribonucleate from calf thymus as substrate. 2. The enzyme is highly active, a measurable effect being obtained at concentrations of less than 0.01 microgram per cc. In highly dilute solution the enzyme is rapidly inactivated, and the use of a protective agent such as gelatin or… 

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