PRISM, a generic large scale proteomic investigation strategy for mammals.

@article{Kislinger2003PRISMAG,
  title={PRISM, a generic large scale proteomic investigation strategy for mammals.},
  author={Thomas Kislinger and Khaled M. Abdel Rahman and Dragan Radulovic and Brian Cox and Janet Rossant and Andrew Emili},
  journal={Molecular & cellular proteomics : MCP},
  year={2003},
  volume={2 2},
  pages={96-106}
}
We have developed a systematic analytical approach, termed PRISM (Proteomic Investigation Strategy for Mammals), that permits routine, large scale protein expression profiling of mammalian cells and tissues. PRISM combines subcellular fractionation, multidimensional liquid chromatography-tandem mass spectrometry-based protein shotgun sequencing, and two newly developed computer algorithms, STATQUEST and GOClust, as a means to rapidly identify, annotate, and categorize thousands of expressed… CONTINUE READING