PRIMO: A primer design program that applies base quality statistics for automated large-scale DNA sequencing.

@article{Li1997PRIMOAP,
  title={PRIMO: A primer design program that applies base quality statistics for automated large-scale DNA sequencing.},
  author={Peng Li and Kenneth Kupfer and Christopher J Davies and David G Burbee and G. A. Evans and Harold R. Garner},
  journal={Genomics},
  year={1997},
  volume={40 3},
  pages={476-85}
}
PRIMO is a computer program that designs walking primers for large-scale DNA sequencing projects. Oligonucleotide primers are predicted automatically, using quality information associated with each base call, eliminating the need for manually viewing the sequence traces or inspecting contig assemblies to determine appropriate locations for primer design. This allows PRIMO to run in batch mode on an arbitrarily large number of templates. For shotgun sequencing, PRIMO reads assembled sequence… CONTINUE READING

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