PRESERVATION OF T2 BACTERIOPHAGE WITH LIQUID NITROGEN.

@article{Meyle1964PRESERVATIONOT,
  title={PRESERVATION OF T2 BACTERIOPHAGE WITH LIQUID NITROGEN.},
  author={J S Meyle and J E Kempf},
  journal={Applied microbiology},
  year={1964},
  volume={12},
  pages={
          400-2
        }
}
There have been few reports on the preservation of viruses at the temperature of liquid nitrogen (-196 C). In this study, factors affecting the survival of phage were observed. Phage lysates in broth did not lose titer after a storage period of 4 weeks in liquid nitrogen. The rate of freezing was not critical, but freezing in nitrogen vapor was not satisfactory. When the virus was partially purified and suspended in buffered saline solution, it rapidly lost titer. Of seven protective agents… CONTINUE READING

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