PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.

@article{Saito1963PREPARATIONOT,
  title={PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.},
  author={H. Saito and K. Miura},
  journal={Biochimica et biophysica acta},
  year={1963},
  volume={72},
  pages={
          619-29
        }
}
  • H. Saito, K. Miura
  • Published 1963
  • Chemistry, Medicine
  • Biochimica et biophysica acta
Abstract Several procedures employing chloroform and phenol for extraction and deproteinization have been explored for the preparation of highly purified transforming DNA from Bacillus subtilis . The recovery, purity, transforming activity and the sedimentation coefficients of the preparations have been compared. The following procedure has been found to give the best results: lysis of cells with lysozyme (EC 3.2.1.17), followed by freezing and thawing, extraction of nucleic acids with a… Expand
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