PPAR-gamma response element activity in intact primary human adipocytes: effects of fatty acids.

@article{Sauma2006PPARgammaRE,
  title={PPAR-gamma response element activity in intact primary human adipocytes: effects of fatty acids.},
  author={Lilian Sauma and Karin G. Stenkula and Preben Kj\olhede and Peter Str{\aa}lfors and Mats S{\"o}derstr{\"o}m and Fredrik H Nystrom},
  journal={Nutrition},
  year={2006},
  volume={22 1},
  pages={60-8}
}
OBJECTIVE We studied the activity and regulation of the peroxisome proliferator-activated receptor-gamma response element (PPRE) in primary human adipocytes. METHODS We transfected primary human adipocytes with a plasmid-encoding firefly luciferase cDNA under control of a PPRE from the acyl-coenzyme A oxidase gene by using our newly developed electroporation-based method. Several fatty acids were added to the fat cells to study potential activation of peroxisome proliferator-activated… CONTINUE READING
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