PPADS selectively antagonizes P2X-purinoceptor-mediated responses in the rabbit urinary bladder.

@article{Ziganshin1993PPADSSA,
  title={PPADS selectively antagonizes P2X-purinoceptor-mediated responses in the rabbit urinary bladder.},
  author={Airat U. Ziganshin and Charles H. V. Hoyle and Xu Bo and G{\"u}nter Lambrecht and Ernst Mutschler and Hans G. B{\"a}umert and Geoffrey Burnstock},
  journal={British journal of pharmacology},
  year={1993},
  volume={110 4},
  pages={1491-5}
}
1. Pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS), an inhibitor of P2X-purinoceptor-mediated responses in rabbit vas deferens, was investigated for its ability to antagonize contractions evoked by alpha,beta-methylene ATP (alpha,beta-MeATP), carbachol and electrical field stimulation in the rabbit urinary bladder detrusor muscle. 2. PPADS. (1-30 microM) caused concentration-dependent inhibition of contractions to the stable P2X-purinoceptor agonist, alpha,beta-MeATP, decreasing… CONTINUE READING

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5 . Radioligand binding studies carried out on rabbit bladder membranes with [ 3H]-alpha , beta - methylene ATP([3H]-alpha , beta - MeATP ) showed that PPADS concentration - dependently inhibited the binding of [ 3H]-alpha , beta - MeATP to P2X - purinoceptors , while the binding of [ 3H]-quinuclidinyl benzilate to muscarinic cholinoceptors was not affected.6 .
5 . Radioligand binding studies carried out on rabbit bladder membranes with [ 3H]-alpha , beta - methylene ATP([3H]-alpha , beta - MeATP ) showed that PPADS concentration - dependently inhibited the binding of [ 3H]-alpha , beta - MeATP to P2X - purinoceptors , while the binding of [ 3H]-quinuclidinyl benzilate to muscarinic cholinoceptors was not affected.6 .
5 . Radioligand binding studies carried out on rabbit bladder membranes with [ 3H]-alpha , beta - methylene ATP([3H]-alpha , beta - MeATP ) showed that PPADS concentration - dependently inhibited the binding of [ 3H]-alpha , beta - MeATP to P2X - purinoceptors , while the binding of [ 3H]-quinuclidinyl benzilate to muscarinic cholinoceptors was not affected.6 .
5 . Radioligand binding studies carried out on rabbit bladder membranes with [ 3H]-alpha , beta - methylene ATP([3H]-alpha , beta - MeATP ) showed that PPADS concentration - dependently inhibited the binding of [ 3H]-alpha , beta - MeATP to P2X - purinoceptors , while the binding of [ 3H]-quinuclidinyl benzilate to muscarinic cholinoceptors was not affected.6 .
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