PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules

@article{Egholm1993PNAHT,
  title={PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules},
  author={M. Egholm and O. Buchardt and L. Christensen and C. Behrens and S. Freier and D. Driver and R. H. Berg and Seog K. Kim and B. Nord{\'e}n and P. Nielsen},
  journal={Nature},
  year={1993},
  volume={365},
  pages={566-568}
}
DNA ANALOGUES are currently being intensely investigated owing to their potential as gene-targeted drugs1–3. Furthermore, their properties and interaction with DNA and RNA could provide a better understanding of the structural features of natural DNA that determine its unique chemical, biological and genetic properties3,4. We recently designed a DNA analogue, PNA, in which the backbone is structurally homomorphous with the deoxyribose backbone and consists of N-(2-aminoethyl)glycine units to… Expand
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A systematic study on the binding properties of this probe towards DNA and RNA complementary strands was carried out by UV and fluorescence spectroscopy, indicating that the PNA probe bind more tightly to RNA rather than to DNA. Expand
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  • P. Nielsen
  • Biology, Medicine
  • Methods in enzymology
  • 1996
TLDR
PNA functions as an artificial transcription factor and presents the potential of developing specific gene activation drugs and the stable and sequence-specific complexes, between PNA and RNA, make PNA an interesting DNA analog in an antisense drug context. Expand
Antisense properties of peptide nucleic acids.
TLDR
Some recent achievements on PNA antisense application are summarized, especially these concerned with whole cell or tissue delivery of the PNA, which suggests that they could potentially serve as efficient antisense or antigene reagents. Expand
Peptide nucleic acid Hoogsteen strand linker design for major groove recognition of DNA thymine bases
TLDR
Computational methods and spatial constraints derived from the experimental structure of a homopyrimidine PNA·DNA-PNA hetero-triplex are used to investigate the influence of linker flexibility on binding interactions of the E-base with thymine and uracil bases in geometry-optimised model systems. Expand
Structural characteristics of cyclopentane-modified peptide nucleic acids from molecular dynamics simulations
A PNA molecule is a DNA strand where the sugar-phosphate backbone has been replaced by a structurally homomorphous pseudopeptide chain consisting of N (2-amino-ethyl)-glycine units. PNA bindsExpand
Conformational and energetic properties of pyrrolidinyl PNA-DNA duplexes: A molecular dynamics simulation
Abstract The binding capability of pyrrolidinyl peptide nucleic acids (PNAs) to DNA have been studied experimentally, also the high affinity and sequence specificity were reported. However, to date aExpand
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