PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space.

Abstract

Centrioles are essential for cilia and centrosome assembly. In centriole-containing cells, centrioles always form juxtaposed to pre-existing ones, motivating a century-old debate on centriole biogenesis control. Here, we show that trans-autoactivation of Polo-like kinase 4 (PLK4), the trigger of centriole biogenesis, is a critical event in the spatial control of that process. We demonstrate that centrioles promote PLK4 activation through its recruitment and local accumulation. Though centriole removal reduces the proportion of active PLK4, this is rescued by concentrating PLK4 to the peroxisome lumen. Moreover, while mild overexpression of PLK4 only triggers centriole amplification at the existing centriole, higher PLK4 levels trigger both centriolar and cytoplasmatic (de novo) biogenesis. Hence, centrioles promote their assembly locally and disfavor de novo synthesis. Similar mechanisms enforcing the local concentration and/or activity of other centriole components are likely to contribute to the spatial control of centriole biogenesis under physiological conditions.

DOI: 10.1016/j.devcel.2015.09.020

Cite this paper

@article{Lopes2015PLK4TC, title={PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space.}, author={Carla M Lopes and Swadhin Chandra Jana and In{\^e}s Cunha-Ferreira and Sihem Zitouni and In{\^e}s Bento and Paulo Duarte and Samuel Gilberto and Francisco Freixo and Ad{\'a}n Guerrero and Maria E Francia and Mariana Lince-Faria and Jorge Carneiro and M{\'o}nica Bettencourt-Dias}, journal={Developmental cell}, year={2015}, volume={35 2}, pages={222-35} }