PLGA microsphere-mediated growth hormone release hormone expression induces intergenerational growth.

Abstract

To improve animal growth, growth hormone-releasing hormone (GHRH) expression vectors that maintain constant GHRH expression can be directly injected into muscles. To deliver the GHRH expression vectors, biodegradable microspheres have been used as a sustained release system. Although administering GHRH through microspheres is a common practice, the intergenerational effects of this delivery system are unknown. To investigate the intergenerational effects of polylactic-co-glycolic acid (PLGA) encapsulated plasmid-mediated GHRH supplements, pCMV-Rep-GHRH microspheres were injected into pregnant mice. Growth and expression of GHRH were measured in the offspring. RT-PCR and immunohistochemistry reveal GHRH expression 3-21 days post-injection. The proportion of GH-positive cells in the GHRH treated offspring was 48.2% higher than in the control group (P < 0.01). The GHRH treated offspring were 6.15% (P < 0.05) larger than the control offspring. At day 49 post-injection, IGF-I serum levels were significantly higher in the treatment group than in the control group. This study confirms that intramuscular expression of GHRH mediated by PLGA microspheres significantly enhances intergenerational growth.

DOI: 10.1080/10495390902945787

Cite this paper

@article{Ren2009PLGAMG, title={PLGA microsphere-mediated growth hormone release hormone expression induces intergenerational growth.}, author={Xiao-hui Ren and Yongliang Zhang and Hu-Ying Luo and Hongyi Li and Song-cai Liu and Ming-jun Zhang and Song-Ying Ouyang and Qian-yun Xi and Qing-yan Jiang}, journal={Animal biotechnology}, year={2009}, volume={20 3}, pages={124-32} }