PKC-dependent phosphorylation of Munc18a at Ser313 in activated RBL-2H3 cells

@article{Adhikari2017PKCdependentPO,
  title={PKC-dependent phosphorylation of Munc18a at Ser313 in activated RBL-2H3 cells},
  author={Pratikshya Adhikari and Hao Xu},
  journal={Inflammation Research},
  year={2017},
  volume={67},
  pages={1-3}
}
Protein Kinase C (PKC) regulates the release of pro-inflammatory compounds from IgE/antigen-activated mast cells by unknown mechanisms. In this study, we show for the first time that PKC inhibitor Ro-03-0432, which inhibits RBL-2H3 exocytosis/degranulation in a concentration-dependent fashion, prevents the phosphorylation of membrane fusion factor Munc18a at Ser 313. Our study provides fresh evidence that PKC-dependent protein phosphorylation may contribute to the intricate regulation of mast… 
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12 Citations

Munc18-2, but not Munc18-1 or Munc18-3, controls compound and single-vesicle–regulated exocytosis in mast cells

MC-regulated exocytosis is required for the anaphylactic response, and Munc 18-2 is the sole Munc18 isoform that mediates membrane fusion during MC degranulation, according to a high-resolution analysis of plasma membrane capacitance.

Imaging of RBL-2H3 Cell Degranulation by Atomic Force Microscopy

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  • Biology
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Munc18-2, but not Munc18-1 or Munc18-3, regulates platelet exocytosis, hemostasis, and thrombosis

Munc18-2, but not Munc18-1 or Munc 18-3, is essential for regulated exocytosis in platelets and platelet participation in thrombosis and hemostasis.

Syntaxin 3, but not syntaxin 4, is required for mast cell–regulated exocytosis, where it plays a primary role mediating compound exocytosis

In vivo model of passive systemic anaphylaxis showed that the residual exocytic function of Stx3-deficient MCs was sufficient to drive a full anaphyactic response in mice, indicating that an Stx other than Stx 3 and -4 is also required for exocytosis in MCs.

Diverse exocytic pathways for mast cell mediators.

This review illustrates existing evidence that highlights the diverse exocytic pathways in mast cells and discusses strategies to delineate these pathways so as to identify unique molecular components which could serve as new drug targets for more effective and specific treatments against mast cell-related diseases.

Curcumin suppresses oxidative stress via regulation of ROS/NF-κB signaling pathway to protect retinal vascular endothelial cell in diabetic retinopathy

It was found that curcumin reduced the reactive oxygen species (ROS) and relieved the apoptosis in RRVECs exposed to the high glucose by flow cytometry, and it was revealed that the increased activity of NF-κB and phosphorylated NF-β in RRvECs induced by high glucose concentration was significantly suppressed byCurcumin.

NLRP3 inflammasome contributes to neurovascular unit damage in stroke

Evidence on this topic suggests that targeting NLRP3-mediated inflammation at multiple levels may provide a new therapeutic strategy to prevent the deterioration of neurovascular units after stroke.

De novo STXBP1 mutation in a child with developmental delay and spasticity reveals a major structural alteration in the interface with syntaxin 1A

  • E. BanneT. Falik-Zaccai M. Linial
  • Biology
    American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics
  • 2020
This study provides a direct link between the outcome of a novel variant in STXBP1 and protein structure and dynamics, and the structural change upon mutation drives an alteration in synaptic function.

Zinc protects against cadmium-induced toxicity in neonatal murine engineered cardiac tissues via metallothionein-dependent and independent mechanisms

Zn exerts protective effects against Cd toxicity for murine ECTs that are partially MT-mediated, and the ability of Zn to reduce Cd uptake provided an additional MT-independent mechanism for reducing Cdoxicity.

Systematically characterize the substance basis of Jinzhen oral liquid and their pharmacological mechanism using UPLC-Q-TOF/MS combined with network pharmacology analysis

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