PIM2 kinase is induced by cisplatin in ovarian cancer cells and limits drug efficacy.

@article{Musiani2014PIM2KI,
  title={PIM2 kinase is induced by cisplatin in ovarian cancer cells and limits drug efficacy.},
  author={Daniele Musiani and Dean E Hammond and Luca Cirillo and Jessica Erriquez and Martina Olivero and Michael J Clague and Maria Flavia Di Renzo},
  journal={Journal of proteome research},
  year={2014},
  volume={13 11},
  pages={4970-82}
}
Platinum-based chemotherapy is widely used to treat various cancers, but many patients ultimately relapse due to drug resistance. We employed phosphoproteomic analysis and functional assays of the response of SK-OV-3 ovarian cancer cells to cisplatin as a strategy to identify kinases as candidate druggable targets to sensitize cells to platinum. A SILAC-based approach combined with TiO2-based phosphopeptide enrichment allowed the direct identification of ERK1/2, p90RSK, and ERBB2 as kinases… CONTINUE READING