PIKK-dependent phosphorylation of Mre11 induces MRN complex inactivation by disassembly from chromatin.

@article{Virgilio2009PIKKdependentPO,
  title={PIKK-dependent phosphorylation of Mre11 induces MRN complex inactivation by disassembly from chromatin.},
  author={Michela Di Virgilio and Carol Y. Ying and J -C. Gautier},
  journal={DNA repair},
  year={2009},
  volume={8 11},
  pages={1311-20}
}
The role of Mre11 phosphorylation in the cellular response to DNA double-strand breaks (DSBs) is not well understood. Here, we show that phosphorylation of Mre11 at SQ/TQ motifs by PIKKs (PI3 Kinase-related Kinases) induces MRN (Mre11-Rad50-Nbs1) complex dissociation from chromatin by reducing Mre11 affinity for DNA. Whereas phosphorylation of Mre11 at these residues is not required for DSB-induced ATM (Ataxia-Telangiectasia mutated) activation, abrogation of Mre11 dephosphorylation impairs ATM… CONTINUE READING