PIASy mediates SUMO-2 conjugation of Topoisomerase-II on mitotic chromosomes.

@article{Azuma2005PIASyMS,
  title={PIASy mediates SUMO-2 conjugation of Topoisomerase-II on mitotic chromosomes.},
  author={Yoshiaki Azuma and Alexei Arnaoutov and Tadashi Anan and Mary Dasso},
  journal={The EMBO journal},
  year={2005},
  volume={24 12},
  pages={2172-82}
}
Here we show that the PIASy protein is specifically required for mitotic modification of Topoisomerase-II by SUMO-2 conjugation in Xenopus egg extracts. PIASy was unique among the PIAS family members in its capacity to bind mitotic chromosomes and recruit Ubc9 onto chromatin. These properties were essential, since PIASy mutants that did not bind chromatin or failed to recruit Ubc9 were functionally inactive. We observed that PIASy depletion eliminated essentially all chromosomal accumulation of… CONTINUE READING
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