PHOELIX: a package for semi-automated helical reconstruction.

Abstract

We describe a set of procedures and algorithms which have been developed to provide an efficient and reliable method for reconstructing a three-dimensional density map from specimens with helical symmetry. These procedures build on the original MRC helical processing suite, with extensions principally developed using the SUPRIM image processing package. Actomyosin is used as a model specimen to demonstrate the utility of this repackaged and expanded set of routines. The time required to complete a three-dimensional map has been reduced from several weeks using traditional manual techniques to a few days. The increased signal/noise provided has allowed for the extraction of additional layer lines not previously identified by manual techniques.

Statistics

0102030'97'99'01'03'05'07'09'11'13'15'17
Citations per Year

160 Citations

Semantic Scholar estimates that this publication has 160 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Whittaker1995PHOELIXAP, title={PHOELIX: a package for semi-automated helical reconstruction.}, author={Michael Whittaker and Bridget Carragher and Ronald A. Milligan}, journal={Ultramicroscopy}, year={1995}, volume={58 3-4}, pages={245-59} }