PGH Synthase and Lipoxygenase Generate Superoxide in the Presence of NADH or NADPH

  title={PGH Synthase and Lipoxygenase Generate Superoxide in the Presence of NADH or NADPH},
  author={Rakesh C. Kukreja and Hermes A. Kontos and Michael L. Hess and Earl F. Ellis},
  journal={Circulation Research},
Purified PGH synthase when acting on arachidonic acid in the presence of reduced nicotinamide-adenine dinucleotide or reduced nicotinamide-adenine dinucleotide 3'-phosphate generated superoxide in burst-like fashion. In eight experiments using different batches of enzyme, the mean ± SE rate of superoxide generation from 100 U of enzyme measured as the superoxide dismutase-inhibitable reduction of cytochrome c was 5.06 ± 0.19 nmol/min in the first minute and 0.35 ± 0.03 nmol/min subsequently… 

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