PFU DNA Polymerase : A Study of Amplification Error Rate and Subsequent Implications for High Fidelity Mutational Spectrometry

  • Sheila Gay Buzzee
  • Published 2007


This thesis tested the Pyrococcus furiosus (PFU) thermostabile DNA polymerase during PCR of the human mitochondrial segment encoding for tRNA glycine (bp 10,009 to 10,216). This segment is of particular interest for highly precise human mutational spectrometry studies, and the enzyme was tested to determine what amplification error rate may be anticipated… (More)

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