PEPCK mRNA localization in proximal tubule and gene regulation during metabolic acidosis.

  title={PEPCK mRNA localization in proximal tubule and gene regulation during metabolic acidosis.},
  author={K D Drewnowsk and M R Craig and S R Digiovanni and John M. McCarty and Anton F. M. Moorman and Wouter H. Lamers and Anton C. Schoolwerth},
  journal={Journal of physiology and pharmacology : an official journal of the Polish Physiological Society},
  volume={53 1},
To identify the nephron segments expressing PEPCK in control and acidotic conditions, PEPCK mRNA was localized in rat kidney using the technique of reverse transcription and polymerase chain reaction (RT-PCR) in individual microdissected S1 S2, and S3 segments of the rat proximal tubule. In controls, the number of tubules expressing PEPCK mRNA was greatest in the S3 segment, moderate in the S2 segment, and least in the S1 segment of the proximal tubule. After NH4Cl feeding, strong signals for… CONTINUE READING

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