PEGylated helper-dependent adenoviral vectors: highly efficient vectors with an enhanced safety profile

@article{Croyle2005PEGylatedHA,
  title={PEGylated helper-dependent adenoviral vectors: highly efficient vectors with an enhanced safety profile},
  author={Maria A. Croyle and Hong Thai Le and Klause D. Linse and Vincenzo Cerullo and Gabriele Toietta and Alain Beaudet and Lucio Pastore},
  journal={Gene Therapy},
  year={2005},
  volume={12},
  pages={579-587}
}
Transgene expression from helper-dependent adenoviral (HD-Ad) vectors is effective and long lasting, but not permanent. Their use is also limited by the host response against capsid proteins that precludes successful gene expression upon readministration. In this report, we test the hypothesis that PEGylation of HD-Ad reduces its toxicity and promotes transgene expression upon readministration. PEGylation did not compromise transduction efficiency in vitro and in vivo and reduced peak serum IL… CONTINUE READING