PCRless library mutagenesis via oligonucleotide recombination in yeast.

@article{Pirakitikulr2010PCRlessLM,
  title={PCRless library mutagenesis via oligonucleotide recombination in yeast.},
  author={Nathan Pirakitikulr and Nili Ostrov and Pamela Peralta-Yahya and Virginia W Cornish},
  journal={Protein science : a publication of the Protein Society},
  year={2010},
  volume={19 12},
  pages={2336-46}
}
The directed evolution of biomolecules with new functions is largely performed in vitro, with PCR mutagenesis followed by high-throughput assays for desired activities. As synthetic biology creates impetus for generating biomolecules that function in living cells, new technologies are needed for performing mutagenesis and selection for directed evolution in vivo. Homologous recombination, routinely exploited for targeted gene alteration, is an attractive tool for in vivo library mutagenesis… CONTINUE READING