PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin.

Abstract

Streptomycetes are high G+C Gram-positive, antibiotic-producing, mycelial soil bacteria. The 8.7-Mb Streptomyces coelicolor genome was previously sequenced by using an ordered library of Supercos-1 clones. Here, we describe an efficient procedure for creating precise gene replacements in the cosmid clones by using PCR targeting and lambda-Red-mediated… (More)

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@article{Gust2003PCRtargetedSG, title={PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin.}, author={Bertolt Gust and Greg L Challis and Kay Fowler and T. Kieser and Keith F. Chater}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={2003}, volume={100 4}, pages={1541-6} }