PCR screening of 3-amino-5-hydroxybenzoic acid synthase gene leads to identification of ansamycins and AHBA-related antibiotic producers in Actinomycetes.

@article{Huitu2009PCRSO,
  title={PCR screening of 3-amino-5-hydroxybenzoic acid synthase gene leads to identification of ansamycins and AHBA-related antibiotic producers in Actinomycetes.},
  author={Z Huitu and W Linzhuan and L Aiming and S Guizhi and Hua Feng and Lv Qiuping and Wang Yuzhen and X Huanzhang and G Qunjie and W Yiguang},
  journal={Journal of applied microbiology},
  year={2009},
  volume={106 3},
  pages={755-63}
}
AIMS The 3-amino-5-hydroxybenzoic acid (AHBA) synthase is one of the essential and unique enzymes for AHBA biosynthesis. The possibility of screening for ansamycin or AHBA-related antibiotic-producing strains from Actinomycetes by targeting an AHBA synthase gene was explored. METHODS AND RESULTS A pair of degenerated primers designed according to the conserved regions of five known AHBA synthases was used to detect AHBA synthase genes within the genomic DNA of Actinomycetes. PCR screening… CONTINUE READING
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