PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants.

Abstract

We have developed a procedure for efficient in situ hybridization of bacterial recombinants created with various types of large-insert cloning vectors. Minimal quantities of crude DNA are amplified and labeled during the degenerate-oligonucleotide-primed polymerase chain reaction. The resulting probes generate high-intensity fluorescent hybridization… (More)

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@article{Kroisel1994PCRPF, title={PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants.}, author={Peter Michael Kroisel and Panos A. Ioannou and Pieter J de Jong}, journal={Cytogenetics and cell genetics}, year={1994}, volume={65 1-2}, pages={97-100} }