PCR-mediated repeated chromosome splitting in Saccharomyces cerevisiae.

  title={PCR-mediated repeated chromosome splitting in Saccharomyces cerevisiae.},
  author={Minetaka Sugiyama and Shigehito Ikushima and Toshimasa Nakazawa and Yoshinobu Kaneko and S. Harashima},
  volume={38 6},
Chromosome engineering is playing an increasingly important role in the functional analysis of genomes. A simple and efficient technology for manipulating large chromosomal segments is key to advancing these analyses. Here we describe a simple but innovative method to split chromosomes in Saccharomyces cerevisiae, which we call PCR-mediated chromosome splitting (PCS). The PCS method combines a streamlined procedure (two-step PCR and one transformation per splitting event) with the CreAoxP… CONTINUE READING