PCR fragmentation of DNA.


A method has been developed to prepare random DNA fragments using PCR. First, two cycles are carried out at 16 degrees C with the Klenow's fragment and oligonucleotides (random primers) with random 3'-sequences and the 5'-constant part containing the site for cloning with the site-specific endonuclease. The random primers can link to any DNA site, and… (More)


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@article{Zheleznaya1999PCRFO, title={PCR fragmentation of DNA.}, author={Lyudmila A Zheleznaya and Valery G. Kossykh and I V Svad'bina and T S Oshman and Nickolay I Matvienko}, journal={Biochemistry. Biokhimiia}, year={1999}, volume={64 4}, pages={373-8} }