PCR for specific detection of haemorrhagic enteritis virus of turkeys, an avian adenovirus.

@article{Hess1999PCRFS,
  title={PCR for specific detection of haemorrhagic enteritis virus of turkeys, an avian adenovirus.},
  author={Markus M Hess and R{\"u}diger Raue and Hafez Mohamed Hafez},
  journal={Journal of virological methods},
  year={1999},
  volume={81 1-2},
  pages={199-203}
}
A hexon gene based PCR was developed for specific amplification of DNA sequences from the haemorrhagic enteritis virus (HEV) of turkeys. The hexon genes of different avian adenoviruses were compared for primer construction. Two regions with low sequence homology between HEV and fowl adenovirus (FAV) hexon genes were selected for primer localisation. In correlation with the known sequence data a fragment of 1647 bp was amplified from a live vaccine and spleens of turkeys suffering from… CONTINUE READING
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