PCR for bioaerosol monitoring: sensitivity and environmental interference.

@article{Alvarez1995PCRFB,
  title={PCR for bioaerosol monitoring: sensitivity and environmental interference.},
  author={Antonia Jos{\'e} Alvarez and Mark P. Buttner and Linda Dale Allen Stetzenbach},
  journal={Applied and environmental microbiology},
  year={1995},
  volume={61 10},
  pages={
          3639-44
        }
}
The PCR technique has potential for use in detection of low concentrations of airborne microorganisms. In this study, the sensitivity of PCR and its susceptibility to environmental interference were assessed with Escherichia coli DH1 as the target organism. Air samples, containing environmental bioaerosols, were collected with AGI-30 samplers and seeded with E. coli DH1 cells. Parallel studies were performed with cells seeded into the sampler prior to collection of air samples to determine the… CONTINUE READING

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The use of solid-phase polymerase chain reaction for the enhanced detection of airborne microorganisms

  • A. J. Alvarez, M. P. Buttner, +5 authors L. D. Stetzenbach
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