PCR fingerprinting of Blastocystis isolated from symptomatic and asymptomatic human hosts

Abstract

Genomic DNA from 16 Blastocystis hominis isolates comprising of eight asymptomatic isolates (A1–A8) and eight symptomatic isolates (S1–S8) was amplified by arbitrarily primed polymerase chain reaction (AP-PCR) using 38 arbitrary 10-mer primers. Six primers (A10, B5, C20, D1, F6, and F10) generated reproducible DNA fingerprints. AP-PCR amplification revealed… (More)
DOI: 10.1007/s00436-006-0177-0

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