PCR-electrospray ionization mass spectrometry: the potential to change infectious disease diagnostics in clinical and public health laboratories.

@article{Wolk2012PCRelectrosprayIM,
  title={PCR-electrospray ionization mass spectrometry: the potential to change infectious disease diagnostics in clinical and public health laboratories.},
  author={Donna M Wolk and Erin J Kaleta and Vicki H. Wysocki},
  journal={The Journal of molecular diagnostics : JMD},
  year={2012},
  volume={14 4},
  pages={295-304}
}
During the past 20 years, microbial detection methods that are genetically based, such as real-time PCR and peptide nucleic acid fluorescent hybridization, coexisted with traditional microbiological methods and were typically based on the identification of individual genetic targets. For these methods to be successful, a potential cause of infection must be suspected. More recently, multiplex PCR and multiplex RT-PCR were used to enable more broad-range testing based on panels of suspected… CONTINUE READING