PCR detection of potentially pathogenic aeromonads in raw and cold-smoked freshwater fish.

@article{GonzlezRodrguez2002PCRDO,
  title={PCR detection of potentially pathogenic aeromonads in raw and cold-smoked freshwater fish.},
  author={Mar{\'i}a-Nieves Gonz{\'a}lez-Rodr{\'i}guez and Jes{\'u}s A Santos and Andr{\'e}s Piquer Otero and M-L Garc{\'i}a-L{\'o}pez},
  journal={Journal of applied microbiology},
  year={2002},
  volume={93 4},
  pages={
          675-80
        }
}
AIMS Development of a PCR assay for detection of aeromonads carrying the hlyA and/or aerA genes in fish. METHODS AND RESULTS The protocol involves an overnight selective enrichment step in tryptic soy broth yeast extract containing 10 microg ml(-1) of ampicillin followed by extraction of DNA and PCR amplification of two haemolysin genes that contribute to the virulence of Aer. hydrophila. This procedure can detect initial populations of 1-10 cfu g(-1) within 24 h in artificially contaminated… CONTINUE READING
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