PCR detection of Neospora caninum in water buffalo foetal tissues

@article{Auriemma2014PCRDO,
  title={PCR detection of Neospora caninum in water buffalo foetal tissues},
  author={Clementina Auriemma and Maria Gabriella Lucibelli and Giorgia Borriello and Esterina De Carlo and Alessandra Martucciello and L. Schiavo and Amalia Gallo and Francesca Isabella Bove and Federica Corrado and Santa Girardi and Maria Grazia Amoroso and Barbara Ďegli Uberti and Giorgio Galiero},
  journal={Acta Parasitologica},
  year={2014},
  volume={59},
  pages={1-4}
}
The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences… CONTINUE READING

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