PCR cloning, heterologous expression, and characterization of isopenicillin N synthase from Streptomyces lipmanii NRRL 3584.

@article{Loke2000PCRCH,
  title={PCR cloning, heterologous expression, and characterization of isopenicillin N synthase from Streptomyces lipmanii NRRL 3584.},
  author={Paxton Loke and Chee Pang Ng and Tiow Suan Sim},
  journal={Canadian journal of microbiology},
  year={2000},
  volume={46 2},
  pages={166-70}
}
A key step which involves the cyclization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine to the bicyclic ring structure of isopenicillin N in the penicillin and cephalosporin biosynthetic pathway, is catalyzed by isopenicillin N synthase (IPNS). In this study, an IPNS gene from Streptomyces lipmanii NRRL 3584 (slIPNS) was cloned via PCR-based homology… CONTINUE READING