PCR-based ordered genomic libraries: a new approach to drug target identification for Streptococcus pneumoniae.

@article{Belanger2002PCRbasedOG,
  title={PCR-based ordered genomic libraries: a new approach to drug target identification for Streptococcus pneumoniae.},
  author={Aimee E. Belanger and Angel Lai and Marcia A Brackman and Donald J Leblanc},
  journal={Antimicrobial agents and chemotherapy},
  year={2002},
  volume={46 8},
  pages={2507-12}
}
Described here are the development and validation of a novel approach to identify genes encoding drug targets in Streptococcus pneumoniae. The method relies on the use of an ordered genomic library composed of PCR amplicons that were generated under error-prone conditions so as to introduce random mutations into the DNA. Since some of the mutations occur in drug target-encoding genes and subsequently affect the binding of the drug to its respective cellular target, amplicons containing drug… CONTINUE READING
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