PCR-based molecular markers for assessment of somaclonal variation in Pinus pinea clones micropropagated in vitro

@article{Cuesta2010PCRbasedMM,
  title={PCR-based molecular markers for assessment of somaclonal variation in Pinus pinea clones micropropagated in vitro},
  author={Candela Cuesta and Ricardo Javier Ord{\'a}s and Armando L. Rodriguez and Bego{\~n}a Fern{\'a}ndez},
  journal={Biologia Plantarum},
  year={2010},
  volume={54},
  pages={435-442}
}
Four different markers [random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), and selective amplified microsatellite polymorphism length (SAMPL)] were applied for evaluating somaclonal variation of micropropagated genotypes of stone pine (Pinus pinea L.). The total number of primers tested was 130, with 223 combinations assayed. A high number of them amplified successfully (178), representing 79.82 % of the total, and the… CONTINUE READING
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