PCR-based method using propidium monoazide to distinguish viable from nonviable Bacillus subtilis spores.

@article{Rawsthorne2009PCRbasedMU,
  title={PCR-based method using propidium monoazide to distinguish viable from nonviable Bacillus subtilis spores.},
  author={Helen Rawsthorne and Christiane Dock and Lee Ann Jaykus},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 9},
  pages={2936-9}
}
This paper describes a molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide. The approach should be valuable in our attempt to employ molecular methods to streamline the evaluation of process validation using bacterial endospores. 
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