PCR-based gene targeting of the inducible nitric oxide synthase (NOS2) locus in murine ES cells, a new and more cost-effective approach

@article{Randolph1996PCRbasedGT,
  title={PCR-based gene targeting of the inducible nitric oxide synthase (NOS2) locus in murine ES cells, a new and more cost-effective approach},
  author={David A. Randolph and James W. Verbsky and Liping Wu Yang and Yifu Fang and Razqallah Hakem and Larry E. Fields},
  journal={Transgenic Research},
  year={1996},
  volume={5},
  pages={413-420}
}
Gene targeting by double homologous recombination in murine embryonic stem (ES) cells is a powerful tool used to study the cellular consequences of specific genetic mutations. A typical targeting construct consists of a neomycin phosphotransferase (neo) gene flanked by genomic DNA fragments that are homologous to sequences in the target chromosomal locus… CONTINUE READING