PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections.

@article{Schild2007PCRbasedDO,
  title={PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections.},
  author={Marc Schild and Christian Gianinazzi and Bruno Gottstein and Niclas Mont Mueller},
  journal={Journal of clinical microbiology},
  year={2007},
  volume={45 2},
  pages={
          564-7
        }
}
We developed a real-time PCR which allowed the highly sensitive detection of Naegleria fowleri in histological brain tissue sections from experimentally infected mice. This genus-specific small-subunit (18S) rRNA gene-based PCR can complement conventional (immuno-) histology for the diagnosis of primary amoebic meningoencephalitis in paraffin-embedded brain necropsy specimens that had been fixed in formalin buffered with phosphate-buffered saline. 
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