PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic

  title={PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic},
  author={E. S. Maskova and Ivana Paulickova},
  journal={Czech Journal of Food Sciences},
A method based on the polymerase chain reaction (PCR) principle was validated for detecting cow's milk in goat and sheep cheeses. DNA was isolated from the cheeses using the isolation kit Invisorb Spin Food I by Invitek Co., designed for the samples of animal origin. The PCR method applied utilizes the sequence of the mitochondrial gene coding cytochrome b which is specific for mammals. It uses the common forward primer and the reverse primer species-specific. After electrophoresis, cow DNA was… 

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