PCR-based detection of canine Leishmania infections in formalin-fixed and paraffin-embedded skin biopsies: elaboration of a protocol for quality assessment of the diagnostic amplification reaction.

Abstract

Diagnosis of the cutaneous form of canine leishmaniosis is mostly performed by histological or immunohistological examination of skin biopsies. In modern histology, the polymerase chain reaction (PCR) has gained increasing importance as a complementary tool to directly demonstrate the presence of parasite DNA in the tissue sections. For the present study, a… (More)

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@article{Mller2003PCRbasedDO, title={PCR-based detection of canine Leishmania infections in formalin-fixed and paraffin-embedded skin biopsies: elaboration of a protocol for quality assessment of the diagnostic amplification reaction.}, author={Norbert M{\"{u}ller and Vanessa Zimmermann and Ursula Forster and Marianne Bienz and Bruno Gottstein and Monika M Welle}, journal={Veterinary parasitology}, year={2003}, volume={114 3}, pages={223-9} }