PCR-based cloning and immunological characterization of Parietaria judaica pollen profilin.

@article{Asturias2004PCRbasedCA,
  title={PCR-based cloning and immunological characterization of Parietaria judaica pollen profilin.},
  author={Juan Andr{\'e}s Asturias and Ignacio Ibarrola and Jos{\'e} Luis Eseverri and M Carmen Arilla and Roberto Gonz{\'a}lez-Rioja and Alberto Mart{\'i}nez},
  journal={Journal of investigational allergology & clinical immunology},
  year={2004},
  volume={14 1},
  pages={43-8}
}
Profilin has been described as an allergen present in pollen of trees, grasses and weeds. Since Parietaria judaica profilin has a molecular mass similar to other Parietaria allergens (Par j 1 and Par j 2) in the 14-10 kDa range, it is difficult to assess the prevalence of profilin by immunoblotting or to obtain sufficient amounts of purified native profilin for investigation and diagnosis. The aim of this study was to identify P. judaica profilin by PCR-based cDNA cloning and to elucidate its… CONTINUE READING
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