PCR assay for the inversion causing severe Hemophilia A and its application.

@article{Liu1999PCRAF,
  title={PCR assay for the inversion causing severe Hemophilia A and its application.},
  author={Jia Liu and Qi-yun Liu and Yan Liang and L{\"u}ya Wang and G Nozary and Bai Xiao and Zi-lu Zhu and Yufeng Zhou and Li-xian Liu and Yuan-zhi Guan and Jianye Zhang and S. S. Sommer},
  journal={Chinese medical journal},
  year={1999},
  volume={112 5},
  pages={419-23}
}
OBJECTIVE To develop a new technique based on long distance polymerase chain reaction (LD-PCR) to replace Southern blotting method to detect Factor VIII (FVIII) gene inversion leading to severe Hemophilia A (HA) and carrier. METHODS Four primers P, Q, A&B were designed and synthesized. P&Q is specific for 5' and 3' flanking regions of F8A1 respectively. A&B is specific for 5' and 3' flanking regions of F8A2/F8A3 respectively. LD-PCR with 3 primers and 3 temprature was set up, optimized and… CONTINUE READING

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